Fig 4.

TRIM22 is active against major human-transmissible IAV strains. (A) A549 cells depleted for TRIM22 (KD-TRIM22) or control cells (KD-CTRL) were infected with A/Brisbane/59/07 (H1N1), (B) NYMC X-181 (H1N1pdm), and (C) A/Wisconsin/67/05 at an MOI of 0.01. Viral replication was measured by titrating infectious particles produced at 24 and 48 h p.i. (D, E, and F). Stably transduced MDCK cells were infected with the same virus strains at an MOI of 0.001. The production of infectious virus was measured by titrating infectious particles produced at 24 and 48 h. Viral titers are expressed as the means ± SD of one representative of two experiments performed in triplicate.