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Schematic representation of the constructs used in this study. The transcription CaMV 35S promoter and the nopaline synthase (NOS) and octopine synthase (OCS) terminators are in gray, and cDNAs are in white. Viral constructs lacking either the PVA 5′- or 3′-UTR were derived from the PVAΔGDD construct. Both monocistronic RLUC and monocistronic GUS were expressed from similar constructs to FLUC and VPg. eIF(iso)4E was expressed from a similar construct as P0 in the pGWB18 vector backbone.
