Fig 5.
P0 has functions distinct from those of other P proteins in PVA infection. (A to C) RLUC activity and RNA accumulation were determined for PVACPmut (A), PVAΔGDD (B), and FLUC (C) during P-protein silencing at 3 DAI. Hairpins were applied 4 days before PVAs. The Agrobacterium OD600 values used for infiltration were as follows: 0.05 for PVACPmut and PVAΔGDD, 0.005 for FLUC, and 0.4 for hairpins. Luciferase activities and RNA amounts were analyzed by using the same samples. (D) Ratio of RNA amount to viral RLUC activity for PVACPmut, calculated by dividing the average relative values calculated from data presented in panel A. *, P < 0.05; **, P < 0.01 (determined by Student's t tests).