Fig 9.
P0 and VPg act synergistically to increase PVA translation. (A and B) VPg and/or P0 was coexpressed with PVAΔGDD (A) and control FLUC (B), and luciferase activity and RNA levels were quantified at 3 DAI. GUS was expressed as a control. The Agrobacterium OD600 values used for infiltration were as follows: 0.05 for PVAΔGDD, 0.005 for FLUC, and 0.25 for P0 and VPg. Agrobacterium carrying the uidA gene (GUS) was used to adjust all infiltrated samples to the same final OD600. (C) GUS/P0/VPg coexpressions, as in panels A and B, in leaves systemically infected with RLUC-tagged wt PVA. The lower leaves were inoculated with PVA, and 7 days later, the systematically infected upper leaves were infiltrated with Agrobacterium carrying GUS/P0/VPg expression cassettes. Viral RLUC activity was determined 3 days later, corresponding to 10 days after PVA inoculation. *, P < 0.05; **, P < 0.01 (determined by Student's t tests).