Fig 6.

Analysis of the vaccine-induced SIV-specific cytotoxic T-cell responses in circulating PBMC and rectal and vaginal MNC at the end of the immunization regimen. (A to C) Granzyme B (GB) production in combination with IFN-γ, TNF-α, and IL-2 was measured in circulating PBMC (A) and rectal (B) and vaginal (C) MNC at the end of the immunization regimen. The variety of total SIV-specific responses in CD4⁺ and CD8⁺ T-cell population is represented as pie graphs for each vaccinated group, and the total mean percentage is shown for each group beneath each pie graph. The total SIV-specific CD4⁺ (left graph) or CD8⁺ (right graph) mean production of granzyme B and its combination with the three cytokines analyzed is represented as bar plots for each vaccinated group. Two-way ANOVA and Bonferroni tests were performed to analyze statistical significance. A P value of < 0.05 was considered statistically significant.