Fig 3.
Comparison of rLm and DNA as priming vaccine vectors for a subsequent rAd5 boosting. Groups of C57BL/6 mice (n = 8/group) received 1010 CFU rLm.SIVgag orally or 50 μg pCMV.SIVgag i.m. and were boosted i.m. with 107 vp rAd5.SIVgag 4 weeks later. Animals that received rAd5.SIVgag alone served as the control (n = 8). At week 7, the magnitude and distribution of vaccine-elicited AL11-specific CD8+ T lymphocyte responses in multiple anatomic sites were determined by Db/AL11 tetramer binding assays. The memory phenotype of the tetramer-positive population was determined based on the expression of CD62L and CD127. Error bars, ±SE. Two-sided t tests were used to compare the means of the results for the rLm-rAd5 group with those for the DNA-rAd5 group (*, P < 0.05; **, P < 0.01). CM, central memory; EM, effector memory; E, effector.