Fig 1.

Effects of AZT, TDF, and RAL on SRV-4 replication. Anti-SRV-4 activities of AZT, TDF, and RAL were evaluated in cell-free transmission (A) and cell-to-cell transmission (B) models. SRV-4-free 293T cells were treated with AZT (400 nM), TDF (10 nM), RAL (150 nM), or vehicle (DMSO). After 4 h, culture media were replaced with fresh medium containing identical concentrations of each drug with replication-competent SRV-4 (A) or with SRV-4-infected 293T cells at a ratio of uninfected to infected cells of 50:1 (B). Culture supernatants were periodically collected, and SRV-4 was concentrated. The viral pellet was lysed, and reverse transcriptase activity derived from SRV-4 was quantified with a standard of known activity to monitor viral production. Data are shown as means and standard deviations obtained from three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; −, P ≥ 0.05 (determined by the Bonferroni test).