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. 2013 Apr;87(8):4322–4329. doi: 10.1128/JVI.03208-12

Table 1.

Susceptibility of VSV-G-pseudotyped luciferase-expressing SRV-4 and related retroviruses/lentiviruses to NRTIs and an INSTI in single-round infectiona

Target cell line and inhibitor Mean EC50 ± SD (μM) (mean % inhibition ± SD)
HIV-1 SRV-4 MoMLV FIV
TE671
    NRTIs
        Thymidine analogs
            AZT 0.018 ± 0.0074 0.042 ± 0.012* 0.019 ± 0.0043 0.029 ± 0.0035
            d4T 0.40 ± 0.10 0.17 ± 0.039 3.7 ± 0.82** 0.52 ± 0.0055
        Inosine analog
            ddI 10 ± 1.7 4.4 ± 1.3** >10 (0) 19 ± 1.7**
        Cytidine analogs
            ddC 5.6 ± 1.1 2.7 ± 0.13** >10 (0)** 3.2 ± 0.50**
            3TC 4.4 ± 0.75 3.9 ± 1.2 >10 (0)** 2.2 ± 0.50*
            FTC 0.48 ± 0.15 0.50 ± 0.082 >10 (0)** 0.35 ± 0.030
        Adenosine analog
            TDF 0.0043 ± 0.00058 0.00080 ± 0.00037** 0.0035 ± 0.0012 0.0015 ± 0.00076**
    INSTI
            RAL 0.0031 ± 0.0015 0.015 ± 0.0065 0.0017 ± 0.00036 0.049 ± 0.00090*
MT-2
    NRTIs
        Thymidine analogs
            AZT 0.037 ± 0.014 0.11 ± 0.037 0.71 ± 0.36* 1.4 ± 0.40**
            d4T 0.50 ± 0.13 2.3 ± 0.44 3.5 ± 0.61 21 ± 6.6**
        Inosine analog
            ddI 3.4 ± 0.70 >10 (42 ± 4.4)* >10 (0)* 16 ± 4.7**
        Cytidine analogs
            ddC 7.2 ± 2.4 0.59 ± 0.45** >10 (0) 3.5 ± 1.1*
            3TC 2.8 ± 1.0 >10 (17 ± 3.3)** >10 (0)** 1.1 ± 0.10**
            FTC 0.52 ± 0.12 3.0 ± 0.40** >10 (0)** 0.22 ± 0.12
        Adenosine analog
            TDF 0.0071 ± 0.0018 0.0016 ± 0.00025** 0.0071 ± 0.00056 0.0039 ± 0.0021
    INSTI
            RAL 0.0033 ± 0.0010 0.0024 ± 0.00068 0.00064 ± 0.00057 0.062 ± 0.032**
a

Antiviral activities of NRTIs and an INSTI against VSV-G-pseudotyped SRV-4, HIV-1, MoMLV, and FIV were determined by using a luciferase assay. Data are shown as means and standard deviations obtained from three or more independent experiments, and statistical analyses were performed (*, P < 0.05; **, P < 0.01; not indicated, P ≥ 0.05 [determined by Dunnett's test against control HIV-1]). EC50s shown as >10 indicate that more than 10 μM drugs is required to block viral infection by 50%. In this case, percent inhibition of viral infection at 10 μM is shown in parentheses and is considered 10 μM for statistical analysis.