Fig 1.

vMyx-Venus/M093 produces fluorescent foci that are similar in size to those produced by the parental viruses. (A) Construction of a recombinant vMyx-Venus/M093. M093 fused with Venus fluorescent protein at the amino terminus (Venus/M093) was generated by inserting the coding sequence of Venus in frame immediately following the start codon of the M093L gene. The arrow indicates the direction of transcription of M093L. aa, amino acids. (B) Characterization of fluorescent foci formed by vMyx-Venus/M093. BSC-40 cell monolayers were infected with the indicated viruses at 37°C for 1 h. At 1 h after infection, inoculum was removed and cell monolayers were overlaid with liquid medium. At 3 days postinfection, fluorescence and phase-contrast images of foci were captured.