Fig 8.

Venus-tagged MYXV or VACV differentially bind CD138⁺ multiple myeloma cells in primary patient bone marrow samples. Human leukocytes were enriched from the primary patient bone marrow samples through a Ficoll gradient. vMyx-Venus/M093 or vVac-Venus/A4 was bound to leukocytes at an MOI of 10.0 on ice for 1 h. After binding for 1 h on ice or infection for 24 h at 37°C, cells were stained with allophycocyanin-conjugated anti-CD138 (A) or anti-CD34 (B), fixed, and analyzed by flow cytometry. Virion binding was determined by flow cytometry.