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Weak interactions of exogenously added ΔN-PAP-III fibers with glial cells. ΔN-PAP-III fibers produced in 25 mm Tris (pH 7.5) containing 150 mm NaCl were added to the culture media of astrocytes, microglia, and Schwann cells, and incubated for 24 h. After fixation, ΔN-PAP-III fibers and cellular shapes were visualized by anti-PAP-III antibody (green) and phalloidin (red) staining, respectively. Scale bar, 50 μm.
