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. 2013 Feb 27;288(15):10241–10253. doi: 10.1074/jbc.M112.443655

FIGURE 4.

FIGURE 4.

SNAI2 induces EMT by repressing miR-203. A, overexpression of SNAI2 in MDA-MB-468 cells induced EMT. The expression of CDH1, VIM, and SNAI2 was examined by immunofluorescence staining. MB468/EV, MDA-MB-468 cells expressing empty vector; MB468/SNAI2, MDA-MB-468 cells expressing SNAI2. Scale bar, 50 μm. B, overexpression of SNAI2 induced VIM and inhibited CDH1 by Western blot. C, SNAI2 repressed miR-203 and miR-200s as determined by TaqMan miRNA assays. D, overexpression of SNAI2 induced endogenous SNAI2 as determined by real time RT-PCR. E, knockdown of SNAI2 induced miR-203 in MDA-MD-231 cells. F, the knockdown of SNAI2 induced miR-203 in Hs578T cells. Error bars represent S.D.