FIGURE 2.

Prostasin depletion induces elevated claudin-2 levels and reduced PKCζ activation. A, representative immunoblot analyses of the tight junction proteins claudin-2 and claudin-1, total PKCζ, and its “active” Thr⁴¹⁰ phosphorylated form (p-PKCζ) present in whole cell lysates on day 6 post-transfection. Average TEERs were as follows: 1148 (control siRNA (siCtl)), 477 (siM2), 463 (siP1), and 367 (siP2) ohms·cm². B, quantification of the relative amount of claudin-2 relative to control siRNA cultures by densitometric scanning of immunoblots. Data are shown as mean ± S.E. C, quantification of the relative ratio of p-PKCζ/total PKCζ by densitometric scanning of immunoblots. Data are shown as mean ± S.E.