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E6AP binds and elongates Lys48-linked tetra-ubiquitin chains. Double-reciprocal plots of initial rates of 125I-ubiquitin conjugation in the presence of the indicated concentrations of UbcH7 were measured as in Fig. 2 under E6AP limiting conditions with 33 nm GST-E6AP in the absence (closed circles) or presence (open circles) of either 6 μm Lys48-linked tetra-ubiquitin (panel A) or 7 μm reductively methylated Lys48-linked tetra-ubiquitin (panel B).
