FIGURE 10.

A and B, effect of removing extracellular Ca²⁺ on the ET-1-induced Ca²⁺ response in PASMCs. Shown are the mean traces and mean values of peak and sustained Ca²⁺ responses activated by ET-1 (10 nm) in 2 mm Ca²⁺-containing solution or in Ca²⁺-free solution (with 1 mm EGTA) (n = eight experiments for each group), which was added 100 s prior to the application of ET-1. C, mean peak of the change in the Ca²⁺ response elicited by 60 mm KCl in the absence (n = 6) and presence (n = 7) of 10 μm Ned-19. D, mean peak of the change in SOCE elicited by readmission of extracellular Ca²⁺ (2 mm) to PASMCs exposed to 10 μm thapsigargin for 20 min under Ca²⁺-free conditions in the absence (n = 9) and presence (n = 9) of 10 μm Ned-19. E, mean peak of the change in the Ca²⁺ response elicited by rapid application of 10 mm caffeine in the absence (n = 7) and presence (n = 7) of 10 μm Ned-19. F, mean peak of the change in [Ca²⁺]_i elicited by Bt₃-InsP₃/AM (20 μm) in the absence (n = 9) and presence (n = 8) of 10 μm Ned-19. The asterisk indicates significant difference from 2 mm Ca²⁺.