FIGURE 2.
NAADP-AM-induced concentration-dependent Ca2+ response in PASMCs. A, mean traces showing the change in [Ca2+]i (Δ[Ca2+]i) evoked by different concentrations of NAADP-AM. B, mean values of peak Δ[Ca2+]i activated by 0.25, 0.50, and 1.00 μm NAADP-AM. Values are mean data from six experiments for each concentration. *, significantly different (p < 0.05) from the control; #, significantly different between 0.50 and 1.00 μm. C, mean traces of Ca2+ transients activated by 1 μm NAADP-AM in the absence and presence of extracellular Ca2+. D, mean values of the peak and sustained increases in [Ca2+]i activated by NAADP-AM (n = 5 for each condition). Values of the sustained response were measured at 500 s.