FIGURE 3.
Effects of bafilomycin A1 and Ned-19 on Ca2+ release induced by NAADP-AM in PASMCs. A, averaged Ca2+ transients activated by 1 μm NAADP-AM with or without preincubation with the vacuolar H+-ATPase inhibitor bafilomycin A1 (3 μm) for 1 h. B, mean values of the peak and sustained increases in [Ca2+]i activated by 1 μm NAADP-AM in the absence and presence of bafilomycin A1 (n = 5). C, mean Ca2+ transients activated by 1 μm NAADP-AM in the absence and presence of the NAADP antagonist Ned-19 (1 and 100 μm; 25-min incubation). D, mean values of the peak and sustained increases in [Ca2+]i activated by 1 μm NAADP-AM in PASMCs with or without pretreatment with Ned-19 (n = six experiments for each group). *, significantly different compared with the control.