FIGURE 4.

Inhibition of SR Ca²⁺ stores upon Ca²⁺ release induced by NAADP-AM in PASMCs. A and B, mean traces of Ca²⁺ transients and mean values of Ca²⁺ responses activated by 1 μm NAADP-AM in PASMCs with (n = 6) or without (n = 7) pretreatment with xestospongin C (10 μm) for 15 min. C and D, mean traces of Ca²⁺ transients and mean values of Ca²⁺ responses activated by 1 μm NAADP-AM in PASMCs with (n = 6) or without (n = 7) pretreatment with ryanodine (50 μm) for 20 min. E and F, mean traces of Ca²⁺ transients and mean values of Ca²⁺ responses activated by 1 μm NAADP-AM in PASMCs with (n = 5) or without (n = 5) pretreatment with thapsigargin (10 μm) for 30 min. *, significantly different compared with the control.