FIGURE 7.

Activation of local Ca²⁺ events by NAADP-AM in ryanodine-treated PASMCs. A, representative confocal line scan images from three different cells. The upper and lower panels show the suppression of NAADP-induced Ca²⁺ sparks in the presence of ryanodine. The lower panel shows the occurrence of repetitive Ca²⁺ events in a single site, leading to a burst in a ryanodine-treated cell. B, a combined figure showing the spark frequency (number of sparks/100 μm/cell) distribution and the averaged global Ca²⁺ transient (F/F₀; red line and symbols) generated from 22 different cells. C, bar graph showing the averaged spark frequency recorded after the application of NAADP in PASMCs with (n = 22) or without (n = 23) pretreatment with ryanodine. D, bar graph showing the averaged global ΔF/F₀ recorded in PASMCs with (n = 22) or without (n = 23) pretreatment with ryanodine at the end of the line scan image (20 s).