FIGURE 8.

Frequency distribution of spatiotemporal properties of Ca²⁺ sparks recorded under steady-state conditions in the absence and presence of NAADP-AM (1 μm). The number of events is expressed as a function of amplitude (F/F₀) (A and B), duration (full duration at half-maximum (FDHM)) (C and D), and spatial spread (full-width at half-maximum (FWHM)) (E and F). The control group consisted of 424 sparks recorded from 71 cells, and the NAADP group consisted of 434 sparks recorded from 58 cells. The box plots show the median and range of each parameter.