FIGURE 9.

Effects of the NAADP antagonist Ned-19, ryanodine, and xestospongin C on ET-1-induced Ca²⁺ response in PASMCs. A and B, mean traces and mean values of peak and sustained Ca²⁺ responses activated by ET-1 (10 nm) in the presence of various concentrations of Ned-19. The sustained Ca²⁺ response is the averaged value between 700 and 800 s. There were six to nine experiments in each group. C and D, mean traces and mean values of Ca²⁺ responses activated by ET-1 (10 nm) in the absence and presence of 50 μm ryanodine (Ryan; red trace) alone or with 1 μm Ned-19 (green trace). There were eight experiments in each group. E and F, mean traces and mean values of Ca²⁺ responses activated by ET-1 (10 nm) in the absence and presence of 10 μm xestospongin C (Xes; red trace) alone or with 1 μm Ned-19 (green trace). There were six to eight experiments in each group. *, significantly different (p < 0.05) from control cells.