TABLE 1.

Primers for conventional and real-time RT-PCR experiments

Gene	NCBI accession no.	Primer	Sequence (5′–3′)	Nucleotide position	Predicted size in bp
Rat tpc1	NM_139332.3	Sensea	GTGCGAGTCACCCGCTGTCC	375–394	122
		Antisensea	GGAAGCCCAGCCACCGCAAT	496–477
		Sense	TCCCTGCGCTCAAGCTCCGA	392–411	309
		Antisense	TGAAAGGCGGCAGCGACTGG	700–681
Rat tpc2	NM_001107566.1	Sensea	GCCCCCTGTCGCTTTGGGAC	1487–1506	107
		Antisensea	GGTGCTGGCTACCACAGCCG	1593–1574
		Sense	TACTCCGGCCCGTGGTCGAT	1870–1889	262
		Antisense	TGCACAGATGCAAGTGTGGATGC	2131–2109

^a Primers used in real-time PCR.