FIGURE 10.
Inhibition of p38 MAPK promotes Fgf10 expression in primary tongue mesenchymal cells. A and B, immunostaining on the tongue of E13.5 control (A) and Wnt1-Cre;Tak1F/F mutant (B) reveals a significantly reduced level of phosphorylated p38 MAPK (p-p38) in the mutant. C and D, immunostaining shows comparable levels of p-Jun in the tongue of E13.5 wild type and Wnt1-Cre;Tak1F/F embryos. E, quantitative RT-PCR shows an approximately 50% enhanced Fgf10 expression in primary tongue mesenchymal cells in the presence of p38 MAPK inhibitor compared with the control. F, Western blotting shows increased FGF10 expression in primary tongue mesenchymal cells in the presence of p38 MAPK inhibitor. *, p < 0.01. Error bars, S.E.