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Cathepsin b-like activity in total extracts from L. donovani, T. cruzi, and T. brucei. Protease activity was measured using the fluorogenic peptide substrate Z-Arg-Arg-AMC after 30 min of incubation in the presence of nicotinamide (Nam). Values were normalized to the activity of the control (100%). Data are mean ± S.D. from three different experiments. Statistical significance was determined by Bonferroni's multiple comparison test. ***, p < 0.001.
