TABLE 1.
Kinetic parameters of glycan transfer to pilin protein acceptor catalyzed by PglL
Each parameter value represents the average of three independent measurements.
| Substratea | Length of acyl chainb and isomerizationc | Km | Vmax | kcat | kcat/Km |
|---|---|---|---|---|---|
| μm | nmol/min·mgr of PglL | 1/min | 1/min·mm | ||
| I | 3 (cis) | 32.7 ± 2.9 | 90.9 ± 8.7 | 0.006 | 0.1 |
| II | 3 (trans) | 31.7 ± 3.2 | 632.2 ± 59.8 | 0.04 | 1.3 |
| III | 2 (trans) | 371.7 ± 4.0 | 63.2 ± 5.4 | 0.004 | 0.01 |
| IV | 4 (trans) | 16.0 ± 2.1 | 719.3 ± 69.2 | 0.04 | 2.7 |
| V | 5 | 1.7 ± 0.9 | 932.7 ± 9.4 | 0.06 | 36.4 |
a The substrates analyzed correspond to different glycan donors constituted by the same glycan portion but different polyisoprenoid chains. The substrates were identified following the same nomenclature as in Fig. 3A.
b Lengths of the polyisoprenoid chains of the substrates according to the number of their constituent isoprenoid units.
c Isomerization of the first isoprenoid subunit bound to pyrophosphate.