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. 2013 Feb 28;288(15):10661–10671. doi: 10.1074/jbc.M113.458000

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Phenotyping of the uba5(ok3364) and ufc1(tm4888) deletion mutants. A, the development of larvae was analyzed 28, 48, and 72 h after hatching. The deletion mutants uba5(ok3364) and ufc1(tm4888) showed a similar delayed development, compared with the wild type. B, uba5(ok3364) and ufc1(tm4888) laid in average 174 and 161 eggs, respectively. Compared with the wild type (average, 293 eggs), the reproduction rate of both mutants was highly significant reduced (p < 0.0001) but did not significantly differ toward each other. C, the adult lifespan of the deletion mutants (average of 14.6 days for uba5(ok3364) and 16.4 days for ufc1(tm4888)) was significantly reduced (p < 0.05) compared with the wild type (average of 20.8 days) but did not differ when compared to each other. D, the survival of the uba5(ok3364) and ufc1(tm4888) was significantly increased under oxidative, heat, and B. thuringiensis stress (p ≤ 0.0001 for juglone, p ≤ 0.001 for cumene, p ≤ 0.01 for heat, and p < 0.05 for B. thuringiensis). The survival of both mutants under cadmium stress was highly significantly reduced (p < 0.001). The mutants did not differ significantly.