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. 2013 Feb 28;288(15):10661–10671. doi: 10.1074/jbc.M113.458000

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Increased Hsp4 expression in the deletion mutant uba5(ok3364) under various stress conditions. The expression of the reporter gene construct hsp4-GFP was visually examined and analyzed under standard and various stress conditions in the deletion mutant uba5(ok3364) and in wild type worms. A consistent increase of the GFP signal could be observed in the deletion mutant under tunicamycin, juglone, heat, and NaCl stress. Since the expression pattern observed under DTT stress was highly variable, a percentage distribution of GFP levels (low, medium, high) is given. The uba5(ok3364) worms also displayed a highly significant increased survival under tunicamycin (97.6% compared with 48% in wild type p < 0.01) and DTT (74.2% survival compared with 4.6% in wild type, p > 0.01) (right panel).