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. 2013 Feb 25;288(15):10849–10859. doi: 10.1074/jbc.M112.446831

FIGURE 1.

FIGURE 1.

nSMase2 regulates cancer cell metastasis. A, the expression level of nSMase2 protein in (upper panel) and secretion level of exosome from (lower panel) MCF10A and MDA-MB-231 cells. The same number of cells was seeded. Error bars are presented as the mean S.E. (n = 3). **, p < 0.005, as compared with MCF10A cells. B, phase-contrast electron microscopy was used to image resuspend exosome pellets. Scale bar, 100 nm. C, bioluminescence quantification of lung metastasis by 4T1-control cells, 4T1-nSMase2-KD cells or 4T1-nSMase2-OE cells. Each error bar is presented as the mean S.E. (n = 4). **, p < 0.005, as compared with 4T1-control cells. D, luciferase activity in the lung, which was used to represent lung metastasis, was recorded for each mouse. Lung images from different mice are shown. Each error bar is presented as the mean S.E. (n = 5). **, p < 0.005, as compared with 4T1-control cells. E, bioluminescence quantification of metastasis by parental MDA-MB-231-D3H2LN (MM231-control) cells, MDA-MB-231-D3H2LN-nSMase2-OE (MM231-nSMase2-OE) cells, or MDA-MB-231-D3H2LN-nSMase2-KD (MM231-nSMase2-KD) cells. Each error bar is presented as the mean ± S.E. (n = 5). *, p < 0.05, as compared with MM231-control cells.