Skip to main content
. 2013 Feb 25;288(15):10849–10859. doi: 10.1074/jbc.M112.446831

FIGURE 5.

FIGURE 5.

Exosomal miR-210 from cancer cells enhanced the angiogenesis in endothelial cells. A, the expression level of miR-210 in the cells (left panel) and exosome (right panel) from miR-210 overexpressing cells and control vector transfected cells. Each error bar is presented as the mean ± S.E. (n = 3). **, p < 0.005, as compared with control. B, Exosome derived from 4T1 cells suppressed the luciferase activity of the sensor vector. HUVECs transfected with an miR-210 sensor vector were used as recipient cells. The recipient cells were incubated in an miR-210-enriched exosome, control exosome, or PBS. After a 1-day incubation, a luciferase reporter assay was performed. The values on the y axis are depicted relative to the normalized luciferase activity of control PBS-treated cells, which is defined as 1. Each error bar is presented as the mean ± S.E. (n = 5). *, p < 0.05; **, p < 0.005, as compared with control. C, exosome did not reduce the luciferase activity of the mutated sensor vector. HUVECs transfected with the mutated miR-210 sensor vector were used as recipient cells. The recipient cells were incubated in an miR-210-enriched exosome, control exosome, or PBS. The luciferase assay was carried out as described above. The values on the y axis are depicted relative to the normalized Renilla luciferase activity of control cells, which is defined as 1. Each error bar is presented as the mean ± S.E. (n = 4). n.s. represents not significant. D, the transfection of anti-miR-210 to HUVECs inhibited the induction of capillary formation by exosomes derived from 4T1 cells. Following transfection with 3 nm of the miR-210 inhibitory molecule (anti-miR-210) or a control molecule (anti-NC), the HUVECs were incubated for 1 day, and these cells were then assessed using the migration assay with miR-210-enriched exosomes, control exosomes, or PBS. A representative image at 48 h after plating is shown, including the quantification of the average number of migrated HUVECs at 48 h after plating. Each error bar is presented as the mean ± S.E. (n = 3). *, p < 0.05; **, p < 0.005 as compared with PBS treatment. The scale bar indicates 100 μm. E, capillary tube formation in endothelial cells seeded onto Matrigel following the addition of miR-210-enriched exosomes, control exosomes, or PBS. A representative image at 16 h after plating is shown, including the quantification of the average number of branches at 16 h after plating. Each error bar is presented as the mean ± S.E. (n = 3). *, p < 0.05; **, p < 0.005 as compared with PBS treatment. The error bar indicates 500 μm.