Table 2.
Primers used for lateral flagellar promoter-lacZ fusion construction
| Promoter | Primer sequencea | Siteb |
|---|---|---|
| fliMLp | 5′-TGCAACAGAGAGCAAACCG-3′ | −486 fliML |
| 5′-CGGGATCCTGAGTTGTTCTCGGTCTG-3′ | +114 fliML | |
| flgMLp | 5′-AGCAAGAGCAATCGGAATC-3′ | −325 flgML |
| 5′-CGGGATCCCATCCGATGTAGTTGCCAAT-3′ | +168 flgML | |
| flgALp | 5′-GGAAGATCTTCCCGGTGATTTTCATATTC-3′ | −494 flgAL |
| 5′-CGGGATCCCGTGCATTCAGCCAGATAG-3′ | +386 flgAL | |
| flgBLp | 5′-CGGGATCCCGTGCATTCAGCCAGATAG-3′ | −419 flgBL |
| 5′-GGAAGATCTTCCCGGTGATTTTCATATTC-3′ | +361 flgBL | |
| maf-5p1 | 5′-GAGCTCTGCGCAAAGAAA-3′ | −521 maf-5 |
| 5′-CGGGATCCTCAAGTGCAAGACCAGAGC-3′ | +339 maf-5 | |
| maf-5p2 | 5′-CGTTGACCCGAGAAGTCA-3′ | −1490 maf-5 |
| 5′-CGGGATCCTCGATCCAGCCTTGAAA-3′ | −523 maf-5 | |
| lafAp | 5′-TGTATGGCACTGGGTTGG-3′ | −1519 lafA |
| 5′-GCGGATCCGGTGGTCATGGAAGCAAA-3′ | +41 lafA | |
| llafBp | 5′-TCTGCTGAAAACCGGTGG-3′ | −553 lafB |
| 5′-TTCTGCGCCTGTAAATTG-3′ | +194 lafB | |
| lafTp) | 5′-TCCCCCGGGTGAGTTTGACACATCACCC-3′ | −732 lafT |
| 5′-CGCGGATCCATGAAACACCAGGACACA-3′ | +53 lafT |
a
Underlined letters show the BamHI restriction site. Double-underlined letters show the BglII restriction site. Italicized letters show the SmaI restriction site.
b
Numbers are numbers of nucleotides upstream (−) or downstream (+) from the start site for the indicated gene.