Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Reference(s) and/or source |
|---|---|---|
| Strains | ||
| A. mimigardefordensis DPN7T | Wild type, DTDP and 3SP utilizing | 19; DSM 17166T, LMG 22922T |
| A. mimigardefordensis Δacd | No growth on DTDP and 3SP | This study |
| E. coli One Shot Mach1-T1R | F− ϕ80lacZΔM15 ΔlacX74 hsdR(rK− mK+) ΔrecA1398 endA1 tonA | Invitrogen, Carlsbad, CA |
| E. coli Top10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) rpsL nupG ϕ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK endA1 | Invitrogen, Carlsbad, CA |
| E. coli BL21(DE3)/pLysS | F ompT hsdSB (rB mB) gal dcm (DE3) pLysS (Cmr) | Novagen, Madison, WI |
| E. coli S17-1 | thi-1 proA hsdR17 (rK− mK+) recA1; tra gene of plasmid RP4 integrated into genome | 25 |
| Plasmids | ||
| pSUP5011 | pBR325 containing the transposon Tn5::mob; Apr Cmr Kmr | 25 |
| pGEM-T Easy | Apr lacPOZ′ | Promega, Madison, WI |
| pGEM-T-Easy::ΔacdDPN7 | Apr lacPOZ′; upstream and downstream flank of acdDPN7 (1.4 kbp) | This study |
| pJQ200mp18Tc | sacB oriV oriT traJ Tcr | 26, 27 |
| pJQ200mp18Tc::ΔacdDPN7 | sacB oriV oriT traJ Tcr; upstream and downstream flank of acdDPN7 (1.4 kbp) | This study |
| pBBR1MCS-5 | Broad-host-range cloning vector; Gmr lacZα | 28 |
| pBBR1MCS–5::acdDPN7 | pBBR1MCS5 containing the acd gene of A. mimigardefordensis DPN7T and a 14–bp upstream region as a 1,220–bp ApaI–PstI fragment; Gmr | |
| pCR2.1–TOPO | Kmr Apr lacZα | Invitrogen, Carlsbad, CA |
| pCR2.1–TOPO::acdDPN7 | Kmr Apr lacZα acdDPN7 | This study |
| pET23a(+) | Apr; C–terminal His6 tag | Novagen, Madison, WI |
| pET23a(+)::acdDPN7 | Apr acdDPN7; expressing AcdDPN7–His6 | This study |
a
For abbreviations used in genotypes of E. coli, see reference 24. Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Gmr, gentamicin resistance; Kmr, kanamycin resistance; Tcr, tetracycline resistance.