Table 3.
Kinetic characterization of desulfinating enzymesa
| Enzyme | Molecular mass (subunit) (kDa) | Substrate | Vmax (μmol min−1 mg−1) | Km (mM) | kcat (s−1) | kcat/Km (s−1 mM−1) | Reference |
|---|---|---|---|---|---|---|---|
| AcdDPN7 | 44.809 | 3SP-CoA | 4.191 | 0.013 | 3.13 | 240.8 | This study |
| CSDE. colic | 43.238 | Cysteine sulfinic acid | 20.00 | 0.24 | 15 | 63 | 53 |
| AspATI33Q/Y214Q/R280Yc | 43.500 | Cysteine sulfinic acid | 0.690b | 0.1 | 0.50 | 5.00 | 54 |
| CystalysinT. denticolac | 46.258d | Cysteine sulfinic acid | 115.4b | 49 | 89 | 1.82b | 55 |
| DszBKA2-5-1 | 40.000 | 2-Phenylbenzene sulfinate | 0.155b | 0.062 | 0.10 | 1.66b | 56 |
| DszBKA2-5-1 | 40.000 | 2′-Hydroxybiphenyl-2-sulfinate | 0.185b | 0.0082 | 0.12 | 15.00b | 56 |
a
CSDE. coli is cysteine sulfinate desulfinase from E. coli. AspATI33Q/Y214Q/R280Y is a cysteine sulfinate desulfinase engineered on the aspartate amino transferase (AspAT) scaffold from E. coli. CystalysinT. denticola is an aspartate aminotransferase-like enzyme isolated from the oral pathogen Treponema denticola. DszBKA2-5-1 is a 2′-hydroxybiphenyl-2-sulfinate desulfinase from Rhodococcus erythropolis strain KA2-5-1.
b
Kinetic parameter has been calculated based on values available in the literature.
c
Pyridoxal 5′-phosphate dependent.
d
Molecular mass was reported in reference 57.