Table 2.

Primers used in this study

Primer	Sequence (5′ to 3′)
Transposon check
nirS-1	ATTTGGCAAGCCACTGGT
nirS-2	GTCGTGCTGGGTGTTGTAGA
norB-1	AATGGCTCCCTGAAATTCG
norB-2	GGAAGCTCAGCAGGTAGGC
nosF-1	CCATGAGCCTGGTCGAGAT
nosF-2	ATGTGGTGGCGATAGAGGTC
Deletion plasmids
pLD314
ΔnapA 5′ flank-1	GGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTAAGCCAGGCTCTTCCTGTTC
ΔnapA 5′ flank-2	CAGTCAGCAGAGGTTTCATGGATTCACGACGGGTGAGGTT
ΔnapA 3′ flank-1	ACCTCACCCGTCGTGAATCCATGAAACCTCTGCTGACTG
ΔnapA 3′ flank-2	CCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATACTCGAAGTTGTGGCAGTTG
pLD263
ΔnarG 5′ flank-1	GGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTGATCTGGGTGCCGTTCATC
ΔnarG 5′ flank-2	TCCAGTTCAGGGTGATCTCCGGCTCTCGTTGCTGGTCTC
ΔnarG 3′ flank-1	GAGACCAGCAACGAGAGCCGGAGATCACCCTGAACTGGA
ΔnarG 3′ flank-2	CCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATGGACCTGGTCGAAGTTCTTG
Complementation plasmid
pAPW1
napA-1	GAATTCTCCAAGCGCTTCAC
napA-2	CAGCACGTCGTAGAGGGTCT