Fig 7.

Secretion analysis of fliA and fliC mutants by Western blotting. (A) Wild-type and fliA mutant cultures were induced with mitomycin C and samples taken after 2.5 h. Proteins in the culture supernatant were precipitated and analyzed. WT, wild type. (B) Cytosolic and culture supernatant proteins of the fliC mutant strain were induced with mitomycin C, and samples were taken at different time points and processed as described in the text. Cytosolic proteins were analyzed at 2.5 h; flagellin control indicates purified flagellin. Lane M, low-range, prestained molecular mass markers (in kDa).