Table 1.
Strains and plasmids used in the study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Plasmids | ||
| pGEM-T Easy vector | 3.0-kb PCR cloning vector, Ampr | Promega |
| pBC SK(+) | 3.4-kb cloning vector, Cmr | Stratagene |
| pJQ200 SK+ | Gmr, sacB, Rp4 mob, R6K ori | 31 |
| pCR-XL-TOPO(+) | 3.5-kb PCR cloning vector, kanr | Invitrogen |
| pXAB | pBCSK(+) containing xciA and ximB loci with 5′-UTR containing promoter | This study |
| pXA | pBCSK(+) containing xciA loci with 5′-UTR containing promoter | This study |
| pFlhBA | pCR-XL-TOPO containing flhBA operon | This study |
| ptac-pBCSK | pBCSK(+) vector containing tac promoter | This study |
| E. coli strains | ||
| DH5α | supE44ΔlacU169 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 Φ80dlacZ ΔM15 | Invitrogen |
| BL21(DE3)/pLysS | F− ompT hsdSB (rB− mB−) gal dcm (DE3)/pLysS (Cmr) | Novagen |
| S.17–1 | Donor host for r6K ori conjugant | MTCC, India |
| pimm/Pimm | ximB with 5′UTR in pGEM-T Easy vector in DH5α | This study |
| X. nematophila strains and plasmids | ||
| ATCC 19061 | Wild type, phase I variant, Ampr | Laboratory stock |
| 19061 xciA | X. nematophila xciA::kan | This study |
| 19061 xciA+ | X. nematophila xciA containing pXAB or pXA | This study |
| 19061 flhA | X. nematophila flhA::cam | 13 |
| 19061 flhA+ | X. nematophila flhA::cam containing 4.16-kb flhBA in pCR-XL-TOPO(+) vector | This study |
| 19061 fliC | X. nematophila fliC::cam | 13 |
| 19061 fliA | X. nematophila fliA::cam | 13 |
| pPS1/PS1 | ptac-pBCSK containing 2.9 kb xciA and ximB genes in xciA-null mutant strain | This study |
| pPS2/PS2 | ptac-pBCSK containing 1.7 kb xciA in xciA-null mutant strain | This study |
| pPS3/PS3 | pPS2 with D535A mutation in xciA-null mutant strain | This study |
| pPS4/PS4 | pPS2 with H538A mutation in xciA-null mutant strain | This study |