Fig 4.

Immunoprecipitation of His-Rbf bound to the sarX promoter. Purified DNA recovered from CYL12646 (His-Rbf expression strain) and CYL12642 (negative-control strain) was PCR amplified with primers sarXdc7 and sarXdc8 (A) or SAO0009F3 and SAO0009R3 (B). Lanes 1, 3, and 4 are from 12646, and lanes 2, 5, and 6 are from 12642. DNA recovered from cell lysates prior to incubation with magnetic beads was used for the reactions shown in lanes 1 and 2. DNA for the reactions shown in lanes 3 and 5 was recovered from incubation of magnetic beads with extracts. Material recovered from mock incubations (extracts without beads) was used for reactions in lanes 4 and 6.