Table 2.
Oligonucleotide primers used in this study
| Primer purpose and name | Sequence |
|---|---|
| Plasmid and strain construction | |
| attB1-sarX-KO1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTCACTAGTATCACAAATAAAGCG |
| sarX-KO2 | CTATGCTTTCGACACTCAATTTCAATTACTAATTTCTCAGTATTCAAAATGTTGC |
| sarX-KO3 | GCAACATTTTGAATACTGAGAAATTAGTAATTGAAATTGAGTGTCGAAAGCATAG |
| attB2-sarX-KO4 | GGGGACCACTTTGTACAAGAAAGCTGGGTCTTTCAATACTTCTCGAATTGTC |
| sarXP1 | GAATTCCACCTTGATATGTATTGC |
| sarXP2 | GGATCCGCCGATAAGCAAATTCTATGC |
| Rbf-hisF | GGATCCGTCATACGCAACGTTTACCACG |
| Rbf-hisR | TTAGTGGTGATGGTGATGGTGTCCACCTTTTTTTCCTATTTTAATTATGTATAACGC |
| Pcp1rbf1 | CAGGTCGACTCTAGAGGATCCGTTTGCAAAATATACAGGGGATTATATATAATGGAAAAC |
| Pcp1rbf2 | TATACAGGGGATTATATATAATGGAAAACAAGAAAGGAAAATAGGAGGTTTATATGGC |
| pcp1rbf3 | ATATTCATTATTAGTAAGTATATGCAAGCATGATTTTGCCATATAAACCTCCTATTTTCC |
| pcp1rbf4 | GACTATGCCATCTTGGCAACGCGTTGTCGCATATTCATTATTAGTAAGTATATGCAAGC |
| rbf39 | ATACATATGGCAAAATCATGCTTGCATATAC |
| rbf41 | ATAGGATCCTTTTGCAATACATATCAAGGTG |
| sarX5 | ACGGCATATGAGAAAGACAATGGCAAAAAAATT |
| sarX6 | TACGGGATCCTCAAATATTTAAAAATTGTTCTACATCTTCAAATAAAGC |
| sarX7 | CATATGAACACCGAAAAACTGGAAACCCTG |
| sarX8 | GGATCCTTAGATGTTCAGGAACTGTTCAACGTCTTC |
| qRT-PCR | |
| sgSarx1 | TGTCCTACTTAAATCTAGCTCATCCATTGCAGTT |
| sgSarx2 | TGAATACTGAGAAATTAGAAACATTGCTTGGCTTCTATAAACA |
| sgicaA2 | GACCTCCCAATGTTTCTGGAACCAACATCC |
| icaA3 | GTCAGACGTTGGCTACTGGGATACTGATATG |
| icaRfor | TACGTTCAATTATCTAATACGCCTGAGGAATTTTCTGGAA |
| icaRrev | AGGATGCTTTCAAATACCAACTTTCAAGAAACAGCAAATATT |
| delta rbf for | ACGCGTTGCCAAGATGGCATAGTCTT |
| delta rbf rev | AGCCTAATTCCGCAAACCAATCGCTA |
| sggyrB3 | GGAATCGGTGGCGACTTTGATCTAGCGAAA |
| sggyrB4 | CGCTCCATCCACATCGGCATCAGTCATAAT |
| EMSAs and immunoprecipitation assays | |
| sgfnbAF2 | GAATATTTGCAAGGGTCAGATCAGGTTAATTTTAGAACTG |
| sgfnbAR2 | CTGTGTGGTAATCAATGTCAAGCGGTGTATTG |
| icaRP1 | CTGCAGGAATTTCTTTACCTACCTTTCGTTAG |
| icaRP2 | GGATCCAACATTTAACACTTTGTTCGTA |
| icaRP3 | GGATCCTCTTGTATTTGTCCGTAAATATTTCCAGAAAATTCC |
| icaAlong 1 | GGATCCGTGTCCCCCTTGAGCCCATC |
| icaAlong 2 | CTGCAGCTTATCCTTCAATTTTTATAACCCCCTACTG |
| icaAdc1 | CTGCAGCCATATGGCTTACAACCTAACTAACGAAAGGTAG |
| icaAdc2 | GGATCCGAAATAGTATTGACTGCGCCAGC |
| icaAdc3 | GGATCCGTGCATCTTGATCAACGATAGTATCTGCATC |
| icaAdc4 | GGATCCTTTCTTCTCGTATTTGAGTGCAAGAACATTAGACA |
| icaAdc5 | GGATCCACATTTATGTCAGGCTTCTTGTTCAATGAATATC |
| icaAdc6 | TTGCAATTTTTTAACTTTTTGCTTTTTTATCCTGTATTTATGTC |
| icaAdc7 | AGATATTCATTGAACAAGAAGCCTGACATAAATG |
| SAO0009F3 | AAGGTGCGCAATTAGAGCGTGCT |
| SAO0009R3 | TCTGCGTTCACAAGCTGTGGTACC |
| sarXdc7 | GAATTCGTTTATAGAAGCCAAGCAATGTTTCTAATTTCTCA |
| sarXdc8 | GGATCCGGAAAAAAATAACACCTTGATATGTATTGCA |