Table 3.
qRT-PCR assays to determine the effects of sarX on expression of icaA and icaR
| Strain [description] | Relative gene expressiona |
|||
|---|---|---|---|---|
| icaA | icaR | rbf | sarX | |
| 6973 [wt (pLI50-rbf)] | 10.65 ± 0.94 | 0.67 ± 0.07 | 6.52 ± 2.70 | 5.24 ± 0.50 |
| 11551 [sarX (pLI50)] | 0.27 ± 0.13 | 1.05 ± 0.04 | ND | <0.001 |
| 11552 [sarX (pLI50-rbf)] | 0.14 ± 0.08 | 1.01 ± 0.21 | 8.00 ± 5.3 | <0.001 |
| 11580 [wt (pLI50-sarX)] | 142 ± 23.8 | 0.39 ± 0.25 | 1.79 ± 0.13 | 9.13 ± 4.64 |
| 11514 [rbf (pLI50-sarX)] | 0.14 ± 0.03 | 1.02 ± 0.06 | <0.001 | 0.053 ± 0.01 |
| 11555 [sarX (pLI50-sarX)] | 152 ± 25.6 | 0.40 ± 0.07 | ND | 19.2 ± 1.41 |
| 11696 [icaADBC] | <0.01 | ND | ND | ND |
| 11688 [icaR (pLI50)] | ND | <0.001 | ND | ND |
| 6968 [rbf (pLI50)] | 0.26 ± 0.01 | ND | ND | 0.01 ± 0.002 |
a
The levels of mRNA (means and standard deviations, in arbitrary units) for each of the indicated genes are expressed relative to expression in the wild-type strain 1112 [wt (pLI50)]. ND, not done.