Table 1.
Strains and plasmids used in this work
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| WAM2267 | UPEC strain CFT073 | 21 |
| WAM2850 | CFT073 + pKD46 | 22 |
| WAM4282 | BW25113 + pKD46 | This study |
| WAM4249 | CFT073 ΔcycA | This study |
| WAM4290 | CFT073 ΔcycA (WAM4249) + pACYC177 | This study |
| WAM4291 | CFT073 ΔcycA (WAM4249) + pcycA | This study |
| WAM4326 | CFT073 gcvB::kan | This study |
| WAM4258 | K-12 strain BW25113 | 19 |
| WAM4372 | BW25113 gcvB::kan | This study |
| GS162 | K-12 thi pheA905 ΔlacU169 araD129 rpsL150 | 23 |
| GS1144 | GS162 ΔgcvB::Ωcm | 13 |
| WAM4420 | BW25113 ΔcycA | This study |
| WAM4418 | WAM4420 + pACYC177 | This study |
| WAM4419 | WAM4420 + pcycA | This study |
| WAM4324 | BW25113 cycA::kan | 20 |
| WAM4316 | BW25113 dadA::kan | 20 |
| WAM4375 | BW25113 pnp::kan | 20 |
| WAM4376 | BW25113 ubiE::kan | 20 |
| WAM4379 | BW25113 ubiF::kan | 20 |
| WAM4388 | BW25113 ubiG::kan | 20 |
| WAM4389 | BW25113 ubiH::kan | 20 |
| WAM4390 | BW25113 ubiX::kan | 20 |
| χ289 | K-12 W1485F− | 11 |
| χ316 | χ289 cycR1 | 11 |
| χ444 | χ316 cycR1 cycR2 | 11 |
| χ453 | χ444 cycR1 cycR2 cycR3 | 11 |
| Plasmids | ||
| pcycA | CFT073 cycA gene cloned into pACYC177 (pWAM3479) | This study |
| pACYC177 | Cloning vector | 24 |
| pKD4 | Template for λ-red kanamycin cassette | 19 |
| pKD13 | Template for λ-red kanamycin cassette | 19 |
| pCP20 | λ-Red Flp recombinase for removal of resistance cassette | 19 |
| pKD46 | λ-Red recombinase expression plasmid | 19 |