Table 2.
DCS sensitivity of E. coli CFT073 and K-12 grown on MOPS glycerol, LB, or human urine agar plates
| Strain | Description | Avg zone of inhibition (mm) ona: |
||
|---|---|---|---|---|
| MOPS glycerol | LB | Human urineb | ||
| CFT073 | Wild type | 51.1 (±1.2) | 14.0 (±1.2) | 22.2 (±0.8) |
| WAM4249 | CFT073 cycA | 13.4 (±0.4) | 13.8 (±0.8) | 21.4 (±0.9) |
| WAM4290 | CFT073 cycA/pACYC177 | 12.9 (±1.0) | 13.7 (±0.7) | 22.6 (±0.8) |
| WAM4291 | CFT073 cycA/pcycA | 53.2 (±1.4) | 13.7 (±0.7) | 22.4 (±0.7) |
| BW25113 | Wild type | 67.1 (±1.3) | 13.1 (±0.3) | ND |
| WAM4420 | BW25113 cycA | 15.2 (±1.5) | 12.9 (±0.2) | ND |
| WAM4418 | BW25113 cycA/pACYC177 | 13.7 (±1.6) | 12.4 (±0.4) | ND |
| WAM4419 | BW25113 cycA/pcycA | 66.1 (±2.2) | 12.4 (±0.2) | ND |
a
DCS sensitivity was determined by antibiotic disk diffusion assay. A 10-μl aliquot from a 24.5 mM solution of DCS was added to each disk. Each data point represents the average zone of growth inhibition and the resulting standard deviations from three experimental replicates.
b
ND, not determined.