Table 5.

Keio strains identified in DCS resistance screen

Straina	Gene description	Function	MICb (μg/ml)	Gen timec (min)
BW25113 dadA	d-Amino acid dehydrogenase	Catalyzes the oxidative deamination of most d-amino acids; involved in alanine metabolism	∼37.5	∼33
BW25113 pnp	RNA polynucleotide phosphorylase/polymerase	3′ to 5′ RNA exonuclease and 3′-terminal RNA oligonucleotide polymerase; involved in RNA maturation and degradation	∼50	∼47
BW25113 ubiE	C-methyltransferase	2-Octaprenyl-6-methoxy-1,4-benzoquinone methylase and S-adenosylmethionine:2-DMK methyltransferase; involved in ubiquinone and menaquinone metabolism	∼75	∼56
BW25113 ubiF	Hydroxylase	2-Octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone hydroxylase; involved in ubiquinone metabolism	∼62.5	∼47
BW25113 ubiG	O-methyltransferase	3-Demethylubiquinone-8 3-O-methyltransferase and 2-octaprenyl-6-hydroxyphenol methylase; involved in ubiquinone metabolism	∼112.5	∼103
BW25113 ubiH	Putative hydroxylase	2-Octaprenyl-6-methoxyphenol hydroxylase; involved in ubiquinone metabolism	∼112.5	∼69
BW25113 ubiX	Decarboxylase	Together with UbiG forms a 3-octaprenyl-4-hydroxybenzoate decarboxylase; involved in ubiquinone metabolism	∼87.5	∼135
BW25113	K-12 wt strain	Parent strain of the Keio collection	∼15	∼35

^aThe gene name represents the Keio strain. BW25113 is the parent strain (wild type) of the Keio collection.

^bThe MIC for each strain was determined in LB broth as described in the text. Three experimental replicates were performed for each strain.

^cGeneration (Gen) times determined during growth in LB broth. Two experimental replicates were performed for each strain and averaged. Generation times for each strain were extrapolated from the log phase of each strain's growth curve.