Fig 2.

Specific activity of purified aconitase proteins. Wild-type and C450S and R741E mutant aconitase proteins were purified from B. subtilis by a four-step purification scheme. Two preparations of each protein were purified from independent cultures, producing six total preparations. The concentrations of the purified proteins were determined by a Bradford assay (Bio-Rad). The individual preparations are presented separately. Values shown are the means and standard deviations from two technical replicates.