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Aconitase binds specifically to the citZ 5′ leader region in vitro. Wild-type aconitase purified from E. coli (His6-Acn, histidine tag cleaved with TEV protease) was mixed in increasing concentrations with in vitro-transcribed citZ 5′ leader RNA (250 nM). The hag RNA was used as a negative control. Reactions were analyzed by polyacrylamide gel electrophoresis and visualized by staining with ethidium bromide.
