Table 4.

Quantitative analysis (qPCR) of episomal forms of LpcGI-2 of L. pneumophila Corby Δlpc1833, Δlpc1884, and Δlpc2123

Circular form of LpcGI-2	Growth phase	Δlpc1833 mutant vs WT			Δlpc1884 mutant vs WT			Δlpc2123 mutant vs WT
		No. of copies		P valuea	No. of copies		P value	No. of copies		P value
		Mean	SEM		Mean	SEM		Mean	SEM
A	E	2.535 × 10−4	7.198 × 10−5	<0.01	1.227	0.448	NS	0.831	0.503	NS
	S	6.318 × 10−4	2.456 × 10−4	<0.01	1.277	0.308	NS	0.674	0.340	NS
B	E	1.137	0.120	NS	2.190	0.495	<0.001	2.693	0.860	<0.001
	S	0.926	0.192	NS	1.234	0.262	NS	1.534	0.578	NS
AB	E	0.868	0.179	NS	1.375	0.313	NS	0.922	0.314	NS
	S	0.886	0.117	NS	1.121	0.265	NS	0.813	0.168	<0.05

^aStatistical significance was determined by Student's t test. NS, not significant (P > 0.05).