Table 5.
PCR analysis of transconjugants, recipient L. pneumophila JR32, and donor strains L. pneumophila Corby WT*, ΔpilT, Δlpc1833, and Δlpc2123h
| Region used in analysis | PCR result |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transconjugants |
L. pneumophila JR32 Smr recipient |
L. pneumophila Corby donor |
|||||||
| WT*a | ΔpilTa | Δlpc1833a | Δlpc2123b | WT* | ΔpilT | Δlpc1833 | Δlpc2123 | ||
| Genes | |||||||||
| lpg0402c | + | + | + | + | + | − | − | − | − |
| lpc1850c | + | + | + | + | − | + | + | + | + |
| lpc2123d | − | + | + | ni | − | + | + | + | ni |
| Circular forms of LpcGI-2d (primers) | |||||||||
| A (2/3) | + | + | − | + | − | + | + | − | + |
| B (1/5) | − | + | + | + | − | + | + | + | + |
| AB (3/5) | − | + | + | + | − | + | + | + | + |
| Primers for analysis of integration in tRNAMete | |||||||||
| 2/6 | − | − | − | − | − | + | + | + | + |
| 2/6f | + | + | + | + | − | − | − | − | − |
| 3/4 | − | − | − | − | − | + | + | + | + |
| 3/4g | + | + | + | + | − | + | + | + | + |
| 5/6 | − | − | − | − | − | + | + | + | + |
| 5/6f | − | + | + | + | − | − | − | − | − |
| 4g/6f | + | + | + | + | + | − | − | − | − |
a
Transconjugant selection only for LpcGI-2-A and LpcGI-2-AB and not for LpcGI-2-B.
b
Transconjugant selection only for LpcGI-2-B and LpcGI-2-AB and not for LpcGI-2-A.
c
Thirty transconjugants were analyzed.
d
Ten transconjugants were analyzed.
e
Three transconjugants were analyzed. tRNA-Met gene lpc1832 or lpg2362.
f
Primer specific for strain JR32 (trnM-lpg2362-U).
g
Primer specific for strain JR32 (trnM-lpg2362-R).
h
All specific primers are listed in Table S1 in the supplemental material. +, detected by PCR; −, not detected by PCR; ni, not investigated; Smr streptomycin-resistant strain.