Fig 6.

Immunogold labeling of C. difficile spores. (A) Weak labeling of the surface of purified spores with anti-CotD antibodies. less than 50% of the spores are labeled, with 0 to 1 particle per spore section. Bar, 200 nm. (B) Strong labeling of the surface of purified spores with anti-CotD antibodies. More than 75% spores are labeled, with as many of 10 particles per spore section. Bar, 100 nm. (C) Labeling of material associated with the outer coat of unpurified spores with anti-CotE^N antibodies. Bar, 200 nm. (D) Labeling of sheet-like material of unpurified spores with anti-CotE^N antibodies. Bar, 200 nm.