Table 2.
Resistance of mutant and wild-type sporesa
| Strain or genotype | % CFU after treatment |
|||||||
|---|---|---|---|---|---|---|---|---|
| Heat (°C) |
Virkon | H2O2 | Chloroform | Toluene | Lysozyme | Ethanol | ||
| 65 | 80 | |||||||
| WT 630Δerm | 113 | 4 | <1 | <1 | 82 | 87 | 91 | 90 |
| cotA::CT555a | 83 | 2 | <1 | <1 | 82 | 83 | 26 | 29 |
| cotB::CT329a | 72 | 3 | <1 | <1 | 80 | 83 | 91 | 91 |
| cotCB::CT220s | 73 | 5 | <1 | <1 | 79 | 89 | 92 | 91 |
| cotD::CT302s | 73 | 4 | <1 | <1 | 79 | 93 | 91 | 93 |
| cotE::CT220s | 77 | 3 | <1 | <1 | 78 | 95 | 92 | 90 |
| cotE::CT1203s | 104 | 3 | <1 | <1 | 87 | 94 | 90 | 88 |
| sodA::CT394s | 112 | 6 | <1 | <1 | 82 | 80 | 95 | 79 |
a
Suspensions of spores were treated with a selection of noxious chemicals or heat, and the numbers of CFU before and after treatment are shown as average percentages for three independent experiments. CFU data and statistical analysis are given in Table S3 in the supplemental material. WT, wild type. Boldface indicates values representing sensitivity.