Table 3.
IEM analysisa
Antibody | Labeling of spore suspension |
|||
---|---|---|---|---|
Unpurifiedb |
Purified, spore surface | |||
Diffuse surface material (exosporium?) | Bulk | SLM | ||
Anti-CotA | No labeling | No labeling | No labeling | Weak |
Anti-CotB | Weak | Strong | Strong | No labeling |
Anti-CotCB | Weak | Weak | Weak | Weak |
Anti-CotD | Weak | No labeling | No labeling | Strong |
Anti-CotE(N) | Strong | Weak | Strong | Strong |
Summary of IEM labeling of wild-type 630 spores; weak, less than 50% spores labeled, 0 to 1 particles/spore section; strong, more than 75% spores labeled, up to 10 particles/spore section.