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Effect of metal ions and EDTA on product formation. Recombinant Re-citrate synthase (Rcs) as isolated from E. coli was preincubated in the DTNB assay mixture together with 0.2 mM metal ions or 0.2 mM EDTA at room temperature for 30 min. Afterwards, the reaction was started by the addition of oxaloacetate.
