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Determination of the stereospecificity of citrate synthase by analysis of [14C]citrate synthesized from [1-14C]acetyl-CoA by Si-citrate synthase of pig heart (A) and the recombinant Re-citrate synthase of S. aciditrophicus (B). The [14C]citrates were converted to acetate and malate in the presence of Si-citrate lyase, malate dehydrogenase, and NADH. Acetate was eluted from the Dowex 1×8 column by 0.2 M formic acid, whereas malate was eluted by 1 M formic acid.
